Vena8 Endothelial

By: Cellix  05/12/2011

The Vena8 Endothelial+™ biochip is ideal for studying cell-cell interaction.  Endothelial cells are easily seeded (via standard pipette) and cultured inside the microcapillaries of the biochip.   As cells flow through the microcapillary of the Vena8 Endothelial+™ biochip, the cell surface receptors may interact with the endothelial cell monolayer which coats the microcapillary walls.   Ideal for use with phase contrast imaging and fluorescence imaging to study cell-cell interactions under conditions mimicking physiological flow.

Vena8 Endothelial+TM biochip

Illustration close-up:

Cell adhesion inside cell-cultured microchannel of Vena8 Endothelial+™ biochip

Example:  Adhesion of THP-1 monocytes at shear stress of 0.5 dyne/cm2 to primary HUVEC cultured in microcapillary

Inflammation - Atherosclerosis: THP-1 monocytes adhering to HUVECs in biochip under flow

Atherosclerosis: primary monocyte adhesion to coronary artery endothelium under shear flow

   

Inflammation: PMA stimulated T-cells adhering to non-stimulated HUVECs under shear flow

Primary Endothelial Cell Culture in Cellix's Vena8 Endothelial+TM biochips. Cells seeded at high density and place in a standard incubator. Monolayer achieved in less than 3 hours! 


Other products and services from Cellix

05/12/2011

Products

Our solutions can be used to measure cell rolling, adhesion, migration and transmigration to antibody-coated or endothelial cell-cultured microcapillaries, producing IC50 curves under shear stress conditions mimicking microcapillary, venous or arterial physiological flow.


05/12/2011

Vena8 Fluoro

As cells flow through the microcapillary of the Vena8 Fluoro+™ biochip, the cell surface receptors may interact with adhesion molecules or ligands which coat the microcapillary walls. The Vena8 Fluoro+™ biochip is ideal for studying cell receptor-ligand interaction and is compatible with confocal microscopy.


05/12/2011

Biochips

Vena8 Fluoro+TM and Vena8TM: Easy to coat microcapillaries with standard pipette with a range of different adhesion molecules including VCAM, ICAM, MAdCAM, fibronectin, vWF, collagen, fibrinogen etc. Suitable for a wide range of cell suspensions including T-cells, monocytes, pbmcs, neutrophils, eosinophils, platelets and whole blood.